Related Experiment Video
Updated: May 22, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
The anaerobic biosynthesis of vitamin B12
Simon J Moore1, Martin J Warren
1School of Biosciences, University of Kent, Giles Lane, Canterbury, Kent, U.K. sjm51@kent.ac.uk
Abstract:
Vitamin B12 (cobalamin) is a cobalt-containing modified tetrapyrrole that is an essential nutrient for higher animals. Its biosynthesis is restricted to certain bacteria and requires approximately 30 enzymatic steps for its complete de novo construction. Remarkably, two distinct biosynthetic pathways exist, which are termed the aerobic and anaerobic routes. The anaerobic pathway has yet to be fully characterized due to the inherent instability of its oxygen-sensitive intermediates. Bacillus megaterium, a bacterium previously used for the commercial production of cobalamin, has a complete anaerobic pathway and this organism is now being used to investigate the anaerobic B12 pathway through the application of recent advances in recombinant protein production. The present paper provides a summary of recent findings in the anaerobic pathway and future perspectives.
Related Concept Videos
Biosynthesis in Bacteria
Amino Acid Catabolism
Anoxygenic Photosynthesis
Microbial Nutrition
Microbes and Methanogenesis
Metabolism of Chemolithotrophs

