Jove
Visualize
Contact Us

Related Concept Videos

Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A single-cell multi-omics assay for simultaneous measurement of vector copy number and protein expression in CAR T cells.

Molecular therapy. Advances·2026
Same author

One Sea, Different Whales: Genomics Sheds Light on a Small Population of Fin Whales.

Genome biology and evolution·2026
Same author

Vitamin D and its role in human milk oligosaccharide production.

The Journal of steroid biochemistry and molecular biology·2026
Same author

A pilot study of maternal vitamin D status and its association with breast milk and infant gut metabolites.

Physiological reports·2026
Same author

Microplastic ingestion affects the lateralised processing of predator stimuli in fish.

Journal of fish biology·2025
Same author

Evaluation of sources of variability in a nitric oxide screening assay for engineered nanomaterials.

Nanomedicine : nanotechnology, biology, and medicine·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 22, 2026

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

Quantitative methods to characterize morphological properties of cell lines.

Annalaura Mancia1, John T Elliott, Michael Halter

  • 1Marine Biomedicine and Environmental Science Center, Medical University of South Carolina, Hollings Marine Laboratory, Charleston, SC 29412, USA.

Biotechnology Progress
|May 24, 2012
PubMed
Summary

Quantitative metrics for cell phenotype are essential for consistent research. This study introduces robust methods to measure cell morphology, interactions, and growth rates, ensuring reliable data across experiments and labs.

More Related Videos

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
10:00

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
08:47

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Related Experiment Videos

Last Updated: May 22, 2026

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
10:00

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
08:47

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Area of Science:

  • Cell Biology
  • Biotechnology
  • Genomics

Background:

  • Descriptive cell characterization terms can cause ambiguity in research data.
  • Genetic markers ensure cell line identification, but quantitative phenotypic metrics are also crucial.
  • Standardized quantitative metrics are needed to compare cell data across different labs and experimental conditions.

Purpose of the Study:

  • To develop and present robust methodologies for quantitatively assessing cell phenotypic characteristics.
  • To establish definitive metrics for cell morphology, cell-cell interactions, and population growth rates.
  • To address the need for unambiguous cell characterization beyond genetic markers.

Main Methods:

  • Quantitative assessment of cell morphology.
  • Measurement of cell-cell interaction dynamics.
  • Analysis of cell population growth rates.
  • Application of methodologies to diverse cell lines including endothelial and fibroblast cells from various species.

Main Results:

  • Development of quantitative metrics that effectively distinguish between different cell lines.
  • Demonstration of phenotypic changes in a cell line over extended passages using quantitative data.
  • Validation of methodologies across multiple cell types (dolphin, bovine, human endothelial cells; mouse fibroblasts).

Conclusions:

  • Quantitative phenotypic metrics provide a reliable way to characterize cell lines.
  • These metrics are vital for ensuring data consistency and comparability in cell-based research.
  • The presented methodologies offer a foundation for standardized cell characterization in scientific studies.