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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 22, 2026

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Facing current quantification challenges in protein microarrays.

Robert Wellhausen1, Harald Seitz

  • 1Fraunhofer Institute for Biomedical Engineering-IBMT, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.

Journal of Biomedicine & Biotechnology
|May 24, 2012
PubMed
Summary
This summary is machine-generated.

Protein microarrays offer a powerful way to study cell proteome variability. These tools enable relative protein quantification and tracking of posttranslational modifications, overcoming limitations of other techniques.

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Last Updated: May 22, 2026

Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Area of Science:

  • Proteomics
  • Molecular Biology
  • Biotechnology

Background:

  • Cell proteomes exhibit significant variability due to posttranslational modifications, splice variants, and polymorphisms.
  • Next-generation sequencing methods provide an incomplete view of a cell's protein status.

Purpose of the Study:

  • To provide an overview of current protein microarray techniques.
  • To discuss recent advancements in relative protein quantification using protein microarrays.

Main Methods:

  • Protein microarray technology.
  • Relative protein quantification strategies.
  • Analysis of posttranslational modifications.

Main Results:

  • Protein microarrays effectively track proteomic changes at the protein level.
  • These arrays are valuable for identifying new protein interaction partners.

Conclusions:

  • Protein microarrays are essential tools for understanding proteome variability.
  • Advancements in protein microarray techniques enhance relative protein quantification capabilities.