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Related Experiment Video

Updated: May 22, 2026

Sulfate Separation by Selective Crystallization with a Bis-iminoguanidinium Ligand

Sulfate Separation by Selective Crystallization with a Bis-iminoguanidinium Ligand

Published on: September 8, 2016

Micelle mediated trace level sulfide quantification through cloud point extraction.

Samrat Devaramani¹, Pandurangappa Malingappa

¹Department of Studies in Chemistry, Central College Campus, Bangalore University, Dr. Ambedkar Veedhi, Bangalore 560 001, India.

Thescientificworldjournal

|May 24, 2012

Summary

A novel cloud point extraction method accurately quantifies trace sulfide levels. This simple protocol utilizes iron reduction and complexation for reliable sulfide determination in environmental water samples.

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Area of Science:

Analytical Chemistry
Environmental Science

Background:

Sulfide quantification is crucial for environmental monitoring.
Existing methods for trace sulfide analysis can be complex or time-consuming.

Purpose of the Study:

To develop a simple and efficient cloud point extraction protocol for trace sulfide quantification.
To optimize the reaction conditions and assess the method's applicability to real-world samples.

Main Methods:

Sulfide reduces iron(III) to iron(II), which then complexes with nitroso-R salt.
The metal-ligand complex is extracted via cloud point formation using cetylpyridinium chloride.
Absorbance is measured at 710 nm after homogenization with ethanol.

Main Results:

Reaction variables including pH, metal ion, ligand, and surfactant concentration were optimized.
The method demonstrated effective extraction and quantification of trace sulfide.
Common interfering ions were evaluated.

Conclusions:

The proposed cloud point extraction method offers a simple and sensitive approach for trace sulfide determination.
The method was successfully applied to landfill leachate and various water samples.
Validation through spiking and comparison with standard methods confirmed its reliability.