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High prevalence of Candida yeast in milk samples from cows suffering from mastitis in poland

Bozena Dworecka-Kaszak1, Alicja Krutkiewicz, Daniel Szopa

  • 1Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, Poland. bozena kaszak@sggw.pl

Thescientificworldjournal
|May 24, 2012
PubMed

Insights

Fungal mastitis in cows is often caused by Candida species. This study identified seven Candida species, with Candida parapsilosis being most common, and used genetic analysis to differentiate them.

Area of Science:

  • Veterinary Mycology
  • Animal Infectious Diseases

Background:

  • Mastitis poses significant economic challenges in the dairy industry.
  • Fungal pathogens, particularly Candida species, are increasingly recognized as etiological agents of mastitis.

Purpose of the Study:

  • To determine the frequency of fungal isolation from mastitic cows.
  • To identify and differentiate fungal species, focusing on the genus Candida.
  • To assess the genetic diversity among isolated Candida strains.

Main Methods:

  • Phenotypic characterization of 55 fungal isolates from 66 milk samples.
  • Genomic DNA isolation and PCR amplification using ITS1 and NL2 primers.
  • Restriction Fragment Length Polymorphism (RFLP) analysis using HpaII and EcoRI enzymes.

Main Results:

  • Seven Candida species were identified based on phenotypic traits, with Candida parapsilosis being the most prevalent.
  • PCR amplification yielded products of varying sizes, indicating genetic differences.
  • RFLP analysis confirmed distinct genetic profiles among species and even among strains with similar phenotypes.

Conclusions:

  • Candida species are common causes of fungal mastitis in dairy cows.
  • Phenotypic identification can be complemented by molecular methods like PCR-RFLP for accurate species differentiation.
  • Genetic analysis revealed significant diversity within and among Candida species isolated from mastitic milk.

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