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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: May 22, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

Molecular beacons in diagnostics.

Sanjay Tyagi, Fred Russell Kramer

    F1000 Medicine Reports
    |May 24, 2012
    PubMed
    Summary
    This summary is machine-generated.

    Molecular beacons are advancing clinical diagnostics by enabling rapid, multiplexed detection of diverse infections and antibiotic-resistant strains. These innovations promise faster and more comprehensive pathogen identification in laboratories.

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    DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

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    Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
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    Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

    Published on: August 6, 2012

    Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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    DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
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    DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

    Published on: February 9, 2024

    Area of Science:

    • Molecular diagnostics
    • Clinical microbiology
    • Biotechnology

    Background:

    • Molecular beacons offer a promising platform for nucleic acid detection.
    • Recent technological advancements are enhancing their diagnostic capabilities.

    Purpose of the Study:

    • To highlight the potential of molecular beacons in clinical diagnostics.
    • To discuss advancements in pathogen detection and antibiotic resistance testing.

    Main Methods:

    • Leveraging technical advances in molecular beacon design.
    • Utilizing principles of molecular beacons for allied technologies.

    Main Results:

    • Enabling multiplexed pathogen detection in single clinical samples.
    • Facilitating rapid identification of antibiotic-resistant strains within hours.

    Conclusions:

    • Molecular beacons are poised to significantly impact clinical diagnostic laboratories.
    • Further development of molecular beacon-based technologies will expand diagnostic applications.