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Is cytochrome P-450scc a transmembrane protein?
S A Usanov1, A A Chernogolov, V L Chashchin
1Protein Chemistry Laboratory, BSSR Academy of Sciences, Minsk, USSR.
FEBS Letters
|November 26, 1990
Summary
Cytochrome P-450scc spans the inner mitochondrial membrane. Antibodies revealed its N- and C-terminal sequences interact with both matrix and cytosolic sides, confirming its transmembrane protein structure.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Cytochrome P-450scc is crucial for steroidogenesis.
- Its precise localization within the inner mitochondrial membrane is key to its function.
Purpose of the Study:
- To determine the topology of cytochrome P-450scc in the inner mitochondrial membrane.
- To elucidate the orientation of its N- and C-terminal regions.
Main Methods:
- Utilized monospecific antibodies against cytochrome P-450scc and its fragments (F1, F2, F3).
- Investigated antibody binding to matrix and cytosolic sides of the inner mitochondrial membrane.
Main Results:
- Antibodies to fragments F1 and F2 bound to both matrix and cytosolic sides.
- Antibodies to fragment F3 exclusively bound to the matrix side.
- These findings support a transmembrane model for cytochrome P-450scc.
Conclusions:
- Cytochrome P-450scc is a transmembrane protein.
- Both N- and C-terminal sequences of cytochrome P-450scc span the inner mitochondrial membrane.