[Screening of the aerobic simultaneous denitrobacteria and its denitrification characteristics]

To screen aerobic heterotrophic denitrifying bacteria from various samples and detect metabolites quantitatively during their denitrification process. The bacteria were screened by limit dilution and crossed separation. Nitrate and nitrite were determined using phenol disulfonic acid and N-(1-naphthyl)-ethylene diamine by ultraviolet spectrophotometry. The N2 was detected by gas chromatography. Six aerobic heterotrophic bacteria were isolated and YY-5 was demonstrated to be the greatest denitrifer. The degradation rate of its hydroxylamine oxidoreductase can reached to 70.9%. Above all, there is a good balance between the removal of total nitrogen and production of N2 in process. No N2O was produced and no nitrate or nitrite were accumulated. The high ability of aerobic heterotrophic bacterium YY-5 for denitrification was screened, which showed higher homology with Acinetobacter genus through 16S rRNA blast.