In vivo screening to determine neurological hazards of nitrogen dioxide (NO2) using Wistar rats

NO(2) is a well-known indoor and outdoor pollutant that may cause adverse health problems. Recently, accumulating but extremely limited evidences show that NO(2) possibly express neurotoxicity and is responsible for various neurological disorders. In the present study, neurological hazard of NO(2) and possible mechanisms were determined in rat pallium following a repetitive inhalation exposure with various concentrations (5, 10 and 20mg/m(3)). After 7-day exposure (6h/day), observable adverse effects were induced encompassing decreased ratio of brain to body weight, mild brain pathology, increased neuronal apoptosis, altered antioxidants (Cu/Zn-SOD, Mn-SOD, GPx and NO) activity and increasing formation of PCO. NO(2) inhalation also induced augment of oncogenes (c-fos, c-jun) levels, and deregulation of apoptosis-related genes (p53, bax and bcl-2) expression. With all above data, the present report provided essential information for the characterization of the neurotoxic hazard of NO(2) and related mechanisms, which is required in response to the general concern about the vulnerability of the neurological system to it.