Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

6.3K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
6.3K
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

79.6K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
79.6K
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

6.3K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
6.3K
Proteomics01:33

Proteomics

9.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.1K
The Significance of Membrane Transport01:44

The Significance of Membrane Transport

40.7K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
40.7K
Membrane Proteins01:30

Membrane Proteins

29.1K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
29.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On the state of protein function prediction: a report on the fourth CAFA challenge.

bioRxiv : the preprint server for biology·2026
Same author

Advances in Protein Function Prediction from the Fifth CAFA Challenge.

bioRxiv : the preprint server for biology·2026
Same author

Whole-genome prediction of bacterial pathogenic capacity on novel bacteria using protein language models with PathogenFinder2.

Bioinformatics (Oxford, England)·2026
Same author

Biocentral: Embedding-based Protein Predictions.

Journal of molecular biology·2026
Same author

Menin inhibition enhances graft-versus-leukemia effects by T-cell activation and endogenous retrovirus induction in AML.

Blood·2025
Same author

Toxin data quality: a critical examination of bacterial exotoxins and animal toxins.

BMC research notes·2025

Related Experiment Videos

Structural genomics plucks high-hanging membrane proteins.

Edda Kloppmann1, Marco Punta, Burkhard Rost

  • 1Department of Bioinformatics and Computational Biology, Technical University Munich, Germany. kloppmann@rostlab.org

Current Opinion in Structural Biology
|May 25, 2012
PubMed
Summary
This summary is machine-generated.

Structural genomics centers are advancing high-throughput determination of integral membrane protein structures. Robotic pipelines and prediction methods aid target selection, but functional annotation requires further development.

Related Experiment Videos

Area of Science:

  • Structural biology
  • Genomics
  • Biochemistry

Background:

  • Integral membrane proteins are crucial but challenging targets in structural biology.
  • Recent establishment of structural genomics centers focuses on these proteins.
  • High-throughput approaches are essential for studying membrane proteins.

Purpose of the Study:

  • To review advances in high-throughput structural determination of integral membrane proteins.
  • To assess the contribution of structural genomics to this field.
  • To identify current challenges and future directions in membrane protein structural biology.

Main Methods:

  • Review of recent literature and structural genomics initiatives.
  • Analysis of success rates in experimental structure determination.
  • Evaluation of computational prediction methods for membrane proteins.

Main Results:

  • High-throughput experimental structure determination of integral membrane proteins is increasingly successful.
  • Structural genomics centers are significantly contributing to this progress via robotic pipelines.
  • Prediction methods for membrane regions and protein comparison are largely sufficient for target selection.

Conclusions:

  • Structural genomics is making significant strides in determining integral membrane protein structures.
  • Current prediction tools are adequate for target identification in membrane protein structural genomics.
  • Improved methods are needed for prioritizing targets within protein families and for function annotation without homology.