Flexural stiffness and composition of the batoid propterygium as predictors of punting ability

Elasmobranchs (sharks, skates and rays) perform at the extremes of locomotion and feeding (i.e. long migrations, high-speed swimming and durophagy). However, very little is known about their cartilaginous skeletal structure and composition in response to loading regimes. In this study, we investigated a batoid (skate and ray) appendicular skeletal element, the propterygium, and its response to forces experienced during punting (benthic pelvic fin locomotion). Punting places a flexural load on this thin, rod-like element. The goals for our study were to determine: (1) the mechanical and compositional properties of the propterygium and (2) whether these properties correlate with punting ability. Using five batoid species of varying punting ability, we employed a three-point bending test and found that propterygium flexural stiffness (33.74-180.16 Nm(2)) was similar to values found in bone and could predict punting ability. Variation in flexural stiffness resulted from differences in mineral content (24.4-48.9% dry mass) and the second moment of area. Propterygia material stiffness (140-2533 MPa) approached the lower limit of bone despite having less than one-third of its mineral content. This drastically lower mineral content is reflected in the radius-to-thickness ratio of the cross-section (mean ± s.e.m.=5.5 ± 0.44), which is comparatively much higher than bony vertebrates. This indicates that elasmobranchs may have evolved skeletal elements that increase buoyancy without sacrificing mechanical properties. Our results highlight the functional parallels between a cartilaginous and bony skeleton despite dramatic compositional differences, and provide insight into how environmental factors may affect cartilaginous skeletal development.