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Published on: February 20, 2012
Development of PCR primer sets for intron 1 of the low-copy gene LEAFY in Davalliaceae
Cheng-Wei Chen1, Li-Yaung Kuo, Chun-Neng Wang
1Botanical Garden Division, Taiwan Forestry Research Institute, Taipei 100, Taiwan.
Premise Of The Study:
Primers were designed for amplifying intron 1 of the single-copy nuclear LEAFY gene for species of Davalliaceae.
Methods And Results:
New primer sets were designed and successfully amplified for intron 1 of the LEAFY gene in 13 species representing the five genera of Davalliaceae. The orthology of these sequences was further confirmed by phylogenetic analyses. Site variation in LEAFY intron 1 sequences across genera of the Davalliaceae and among accessions of the Humata repens complex were 18% and 8%, respectively. Such variation was greater than that for the cpDNA atpB-rbcL intergenic spacer region across the same taxa and accessions.
Conclusions:
Using our newly designed primers, intron 1 of the LEAFY gene could be amplified for all species tested. In addition, this single-copy, biparentally inherited, and quickly evolving region showed considerable potential for addressing infraspecific-level questions.
