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Published on: August 18, 2019
Development of microsatellite markers in Parakmeria nitida (Magnoliaceae)
Jia-Min Li1, Qing-Qing Li, Long-Qian Xiao
1Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, People's Republic of China.
Premise Of The Study:
We developed microsatellite makers for Parakmeria nitida to investigate its population structure and conservation genetics.
Methods And Results:
A total of 25 microsatellite primer pairs were developed using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, and polymorphism was assessed in three natural populations of P. nitida. Among these markers, 11 were monomorphic and 14 showed polymorphism.
Conclusions:
These markers are potentially useful for future population genetic analyses of P. nitida and will serve as an important tool for conservation efforts.
