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Updated: May 22, 2026

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
Published on: June 25, 2015
Lack of interference with immunogenicity of a chimeric alphavirus replicon particle-based influenza vaccine by
Yasushi Uematsu1, Michael Vajdy, Ying Lian
1Novartis Vaccines and Diagnostics S.r.l., Siena, Italy. yasushi.uematsu@novartis.com
Abstract:
Antivector immunity has been recognized as a potential caveat of using virus-based vaccines. In the present study, an alphavirus-based replicon particle vaccine platform, which has demonstrated robust immunogenicity in animal models, was tested for effects of antivector immunity on immunogenicity against hemagglutinin of influenza virus as a target antigen and efficacy for protection against lethal challenge with the virus. Chimeric alphavirus-based replicon particles, comprising Venezuelan equine encephalitis virus nonstructural and Sindbis virus structural components, induced efficient protective antibody responses, which were not adversely influenced after multiple immunizations with the same vector expressing various antigens.
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