Measurement techniques for melanoma: a statistical comparison
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Updated: Oct 11, 2026

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Published on: February 8, 2018
C J Calder1, A P Campbell, S R Plastow
1Department of Histopathology, Wycombe General Hospital, Buckinghamshire.
Abstract:
Inter- and intra-observer variation in measuring the depth of invasion of malignant melanomas was assessed using three different techniques: eye-piece graticule, stage Vernier, and projection image analysis. Significant variation was found for all methods but was least pronounced with the stage Vernier. It is recommended that this should be the preferred technique for routine use.
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