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Related Experiment Videos

Evaluation of a prototype for a reference platelet counter.

S M Lewis1, R M Rowan, F Kubota

  • 1Royal Postgraduate Medical School, London.

Journal of Clinical Pathology
|November 1, 1990
PubMed
Summary
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A new semiautomated particle counter prototype offers a reliable reference for platelet counts. This instrument minimizes errors, ensuring accurate values for automated blood cell count preparations.

Area of Science:

  • Hematology
  • Biomedical Instrumentation
  • Particle Counting Technology

Background:

  • Accurate platelet counting is crucial for diagnosing and managing various hematological conditions.
  • Existing automated blood cell counters require reliable reference materials for calibration and validation.
  • Development of a precise reference counter is essential for standardizing platelet value assignments.

Purpose of the Study:

  • To develop a semiautomated single channel aperture-impedance particle counter as a prototype.
  • To establish a reference platelet count for value assignment in reference preparations.
  • To evaluate the performance of the developed counter against established protocols.

Main Methods:

  • Designed a semiautomated single channel aperture-impedance particle counter with sheath flow.

Related Experiment Videos

  • Utilized a 50-micron diameter, 60-micron length aperture to minimize flow and coincidence errors.
  • Employed fixed volume and red blood cell:platelet ratios to prevent dilution errors.
  • Assessed the counter according to the International Committee for Standardization in Hematology (ICSH) protocol.

    • Main Results:

    • The counter demonstrated a high level of linearity and precision.
    • Accurate coincidence correction was achieved.
    • Controlled volume, stability, and negligible carryover were observed.
    • The instrument met the rigorous evaluation criteria of the ICSH protocol.

    Conclusions:

    • The developed semiautomated particle counter serves as a viable prototype for reference platelet counting.
    • The instrument's design effectively minimizes common sources of error in particle counting.
    • This technology can contribute to improved accuracy and standardization in automated hematology.