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Related Experiment Videos

Lectin typing of Campylobacter isolates.

N O'Sullivan1, J Benjamin, M B Skirrow

  • 1Department of Microbiology, Worcester Royal Infirmary.

Journal of Clinical Pathology
|November 1, 1990
PubMed
Summary

Lectin typing of Campylobacter isolates revealed 23 distinct patterns, offering a simple and economical method for bacterial identification. This technique shows promise as a complementary or standalone typing scheme for Campylobacter species.

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Area of Science:

  • Microbiology
  • Bacteriology
  • Immunology

Background:

  • Campylobacter species are significant foodborne pathogens.
  • Accurate identification and typing of Campylobacter isolates are crucial for epidemiological surveillance and control.
  • Existing typing methods like serotyping can be complex or limited in discriminatory power.

Purpose of the Study:

  • To evaluate the utility of lectin agglutination as a novel typing method for Campylobacter jejuni, C. coli, C. fetus, and C. laridis.
  • To determine the reproducibility and discriminatory potential of lectin typing.
  • To assess the correlation between lectin types and established serotypes.

Main Methods:

  • Agglutination reactions were performed on 376 Campylobacter isolates using a panel of five lectins: Arachis hypogaea, Bauhinia purpurea, Solanum tuberosum, Triticum vulgaris, and Wisteria floribunda.
  • Lectin types were recorded, and patterns were assessed for consistency and reproducibility.
  • Correlation analysis was conducted between lectin types and Penner/Lior serotypes.

Main Results:

  • Twenty-three distinct lectin agglutination patterns (lectin types) were identified among the 376 isolates.
  • Lectin typing demonstrated high consistency and reproducibility, with only 4.5% of isolates being untypable due to autoagglutination.
  • Certain lectin types were species-specific or predominant, while others were shared across species. Notably, 42% of C. jejuni and 35% of C. coli isolates belonged to lectin type 4.
  • No apparent correlation was observed between lectin type and serotype.

Conclusions:

  • Lectin typing is a simple, economical, and reproducible method for differentiating Campylobacter species.
  • It can serve as a valuable adjunct to existing serotyping schemes or, with further development, as a standalone typing method.
  • This technique is suitable for use in non-specialist laboratories, enhancing Campylobacter surveillance capabilities.

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