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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Transcriptomics: mRNA and alternative splicing.

Alex Sánchez-Pla1, Ferran Reverter, M Carme Ruíz de Villa

  • 1Departament d'Estadística, Universitat de Barcelona, Spain. alex.sanchez@vhir.org

Journal of Neuroimmunology
|May 26, 2012
PubMed
Summary
This summary is machine-generated.

Transcriptomics, using microarrays and RNA-sequencing, aids biomarker discovery in diseases like multiple sclerosis (MS). These high-throughput technologies identify potential biomarkers for complex conditions.

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Last Updated: May 22, 2026

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Transcriptomics is a key method for identifying disease biomarkers.
  • High-throughput transcriptomic technologies include microarrays and next-generation sequencing (NGS).
  • Multiple sclerosis (MS) research has utilized transcriptomics to understand its pathogenesis.

Purpose of the Study:

  • To review transcriptomic technologies for biomarker discovery.
  • To compare microarrays and RNA-sequencing (RNA-seq) for biomarker identification.
  • To highlight the application of transcriptomics in diseases like MS.

Main Methods:

  • Description of microarray technology for transcriptomic analysis.
  • Overview of next-generation sequencing (NGS), specifically RNA-seq.
  • Review of existing transcriptomic datasets for biomarker discovery.

Main Results:

  • Microarrays have generated extensive datasets for various diseases, including MS.
  • RNA-seq offers comparable power to microarrays.
  • RNA-seq extends capabilities to analyze alternative splicing and discover novel transcripts.

Conclusions:

  • Transcriptomics, through microarrays and RNA-seq, is crucial for biomarker discovery.
  • RNA-seq provides advanced capabilities beyond traditional microarrays.
  • These technologies are vital for understanding complex diseases and identifying new therapeutic targets.