Related Experiment Video
Updated: May 22, 2026

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
Published on: February 18, 2020
Regulation of CREB signaling through L-type Ca2+ channels by Nipsnap-2
Joel M Brittain1, Yuying Wang, Sarah M Wilson
1Program in Medical Neuroscience, Paul and Carole Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
Abstract:
A recent study identified Nipsnap1 as an auxiliary protein inhibiting TRPV6 ion channel activity. Based upon this finding, we investigated the role of Nipsnap1, and the closely related Nipsnap2, in Ca(2+) channel regulation. Here, we find that overexpression of Nipsnap2 caused a 45% increase in currents though L-type Ca(2+) channels in a neuronal cell line, while siRNA knockdown of Nipsnap2 greatly reduced L-type currents. The increased influx through L-type Ca(2+) channels due to Nipsnap2 overexpression led to increased phosphorylation of the transcription factor cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) along with enhanced expression of several transcription factors and CREB target genes. These experiments highlight a novel role of Nipsnap2 in transcriptional regulation via L-type Ca(2+) channels.
More Related Videos
10:03Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
Published on: August 10, 2014
11:33Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
Published on: March 22, 2019
Related Concept Videos
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Regulation of Nuclear Protein Sorting
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
GPCRs Regulate Adenylyl Cylase Activity
Two...
IP3/DAG Signaling Pathway
cAMP-dependent Protein Kinase Pathways