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Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Imaging Biological Samples with Optical Microscopy

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Total Internal Reflection Fluorescence Microscopy

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Phase Contrast and Differential Interference Contrast Microscopy

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Related Experiment Video

Updated: May 22, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Simultaneous optically sectioned fluorescence and optical coherence microscopy with full-field illumination.

Houssine Makhlouf¹, Karen Perronet, Guillaume Dupuis

¹Laboratoire Charles Fabry, CNRS UMR 8501, Institut d’Optique Graduate School, University Paris-Sud, Palaiseau, France.

|May 26, 2012

Summary

A new dual modality instrument combines full-field optical coherence microscopy (FF-OCM) and fluorescence microscopy for enhanced biological tissue screening. This approach provides complementary microarchitecture and cellular-level details for improved disease diagnosis.

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Published on: August 4, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

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Last Updated: May 22, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Biomedical Imaging
Optical Microscopy
Diagnostic Technologies

Background:

Traditional microscopy methods often lack the ability to provide both microarchitectural and specific biochemical information simultaneously.
Optical coherence microscopy and fluorescence microscopy offer complementary imaging capabilities but are typically used as separate systems.

Purpose of the Study:

To develop and evaluate a novel dual modality instrument integrating full-field optical coherence microscopy (FF-OCM) and optically sectioned fluorescence microscopy.
To leverage the strengths of both FF-OCM and fluorescence microscopy for comprehensive biological tissue analysis.

Main Methods:

Implementation of a novel dual modality instrument combining FF-OCM and structured illumination fluorescence microscopy.
Utilizing broad field illumination for simultaneous acquisition of the entire field of view, avoiding raster scanning.
Achieving optical sectioning through coherence gating in FF-OCM and structured illumination in fluorescence microscopy.

Main Results:

The dual modality instrument successfully acquired complementary image data from biological tissues.
FF-OCM provided detailed tissue microarchitecture, while fluorescence microscopy offered cellular-level resolution of specific features using contrast agents.
Demonstrated the potential for combined morphological and biochemical feature analysis.

Conclusions:

The integrated FF-OCM and fluorescence microscopy system offers a powerful tool for biological tissue screening.
Complementary imaging data can significantly enhance the understanding of cellular functions and improve disease diagnosis.
This dual modality approach represents a significant advancement in preclinical and diagnostic imaging.