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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 22, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Analytical challenges and technical limitations in assessing circulating miRNAs.

Anna Zampetaki1, Manuel Mayr

  • 1King's British Heart Foundation Centre, King's College London, London, UK. anna.zampetaki@kcl.ac.uk

Thrombosis and Haemostasis
|May 26, 2012
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) show promise as biomarkers for cardiovascular diseases and intercellular communication. This review details the technologies and analytical challenges for accurately quantifying circulating miRNAs.

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Last Updated: May 22, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

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Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
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Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiovascular Research

Background:

  • MicroRNAs (miRNAs) are increasingly recognized for their roles in cardiovascular diseases (CVDs).
  • These small non-coding RNAs are implicated in intercellular communication.
  • Accurate detection and quantification of circulating miRNAs are crucial for diagnostic and prognostic applications.

Purpose of the Study:

  • To review the technical aspects of circulating miRNA detection and quantification.
  • To discuss the analytical challenges associated with miRNA profiling.
  • To highlight advancements in sensitive and specific miRNA detection platforms.

Main Methods:

  • Review of current technological approaches for miRNA quantification.
  • Analysis of methodologies for circulating miRNA detection.
  • Discussion of challenges in miRNA profiling.

Main Results:

  • Multiple technological platforms exist for miRNA detection, offering improved sensitivity and specificity.
  • Analytical challenges include sample handling, RNA extraction, and assay validation.
  • Standardization of methods is essential for reliable results.

Conclusions:

  • Circulating miRNAs hold significant potential as biomarkers in cardiovascular medicine.
  • Technological advancements are enhancing the ability to profile miRNAs.
  • Addressing analytical challenges is key to realizing the clinical utility of miRNA biomarkers.