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Updated: May 22, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection
Published on: February 17, 2017
The activity of the TRP-like channel depends on its expression system
Shaya Lev1, Ben Katz, Baruch Minke
1Department of Medical Neurobiology, Institute of Medical Research Israel-Canada, Hebrew University, Jerusalem, Israel.
Abstract:
The Drosophila light activated TRP and TRPL channels have been a model for TRPC channel gating. Several gating mechanisms have been proposed following experiments conducted on photoreceptor and tissue cultured cells. However, conclusive evidence for any mechanism is still lacking. Here, we show that the Drosophila TRPL channel expressed in tissue cultured cells is constitutively active in S2 cells but is silent in HEK cells. Modulations of TRPL channel activity in different expression system by pharmacology or specific enzymes, which change the lipid content of the plasma membrane, resulted in conflicting effects. These findings demonstrate the difficulty in elucidating TRPC gating, as channel behavior is expression system dependent. However, clues on the gating mechanism may arise from understanding how different expression systems affect TRPC channel activation.
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