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Related Experiment Video

Updated: May 22, 2026

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys₂-His₂ Zinc-finger Domains

Published on: March 25, 2015

Encapsulating zinc(II) within a hydrophobic cavity.

Deanna L Miller¹, Connie C Lu

¹Department of Chemistry, 207 Pleasant St SE, Minneapolis, MN, USA.

Dalton Transactions (Cambridge, England : 2003)

|May 26, 2012

Summary

A novel macrobicyclic ligand was synthesized to bind zinc ions (Zn2+). This ligand features a unique triamido(amine) structure and a hydrophobic channel, directing metal coordination internally.

Area of Science:

Coordination Chemistry
Supramolecular Chemistry
Organic Synthesis

Background:

Designing ligands with specific metal-binding properties is crucial in chemistry.
Macrobicyclic ligands offer unique structural control over metal ion coordination.

Purpose of the Study:

To synthesize and characterize a novel macrobicyclic ligand.
To investigate the coordination behavior of the ligand with zinc ions (Zn2+).

Main Methods:

Organic synthesis of the macrobicyclic ligand.
Spectroscopic characterization (e.g., NMR, Mass Spectrometry).
X-ray crystallography to determine the structure of the metal complex.

Main Results:

Successful preparation of the macrobicyclic ligand.

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Demonstration of selective binding of Zn(2+) within the ligand's internal cavity.

Structural analysis revealed a triamido(amine) coordination motif and a hydrophobic channel.

Conclusions:

The new macrobicyclic ligand effectively binds Zn(2+) in a defined internal site.
The ligand's structure, featuring a hydrophobic channel, influences metal ion accessibility and coordination.
This work provides a new platform for developing functional metal complexes.