Related Experiment Video
Updated: May 22, 2026
Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
Published on: May 4, 2020
A very sensitive flow-injection spectrophotometric determination method for iron (II) and total iron using 2', 3, 4',
Adem Asan1, Rukiye Aydin, Deniz Karsli Semiz
1Department of Chemistry, Faculty of Science, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey. aasan@omu.edu.tr
Abstract:
In this study, an ultra-sensitive and highly selective, rapid flow-injection spectrophotometric method for the determination of iron (II) and total iron has been proposed. The method was based on the reaction between iron (II) and 2', 3, 4', 5, 7-pentahydroxyflavone in slightly acidic solution with a strong absorption at 415 nm. The carrier solution used was 1 × 10(-5) M 2', 3, 4', 5, 7-pentahydroxyflavone in 0.1 M HAc/Ac(-) buffer solution at pH 4.5. Parameters that affect simultaneously the determination of iron (II) and interfering ions were tested. The relative standard deviation for the determination of 50 μg L(-1) iron (II) was 0.85 % (n = 10), and the limit of detection (blank signal plus three times the standard deviation of the blank) was 3 μg L(-1), both based on injection volumes of 20 μL. The method has been successfully applied to the determination of iron (II) and total iron in water samples and ore samples. The method was verified by analysing a certified reference material Zn/Al/Cu 43XZ3F.
