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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
Published on: June 3, 2016
Epigenetic modulation by TFII-I during embryonic stem cell differentiation
Dashzeveg Bayarsaihan1, Aleksandr V Makeyev, Badam Enkhmandakh
1Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, School of Dentistry, University of Connecticut, Farmington, CT 06030, USA. dashzeveg@uchc.edu
Journal of Cellular Biochemistry
|May 26, 2012
Summary
Transcription factor TFII-I is crucial for vertebrate embryogenesis, priming gene loci in stem cells. It influences epigenetic control of stem cell differentiation by interacting with bivalent chromatin.
Area of Science:
- Developmental Biology
- Epigenetics
- Molecular Biology
Background:
- Transcription factor TFII-I is vital for early vertebrate development.
- TFII-I binding sites have been identified across the genome in mouse embryonic stem cells and tissues.
Purpose of the Study:
- To summarize current knowledge on TFII-I's role in the epigenetic regulation of stem cell differentiation.
- To highlight TFII-I's interaction with bivalent chromatin in lineage-specific genes.
Main Methods:
- Genome-wide mapping studies using ChIP-seq and ChIP-chip.
- Analysis of TFII-I binding regions and their co-localization with epigenetic marks.
Main Results:
- TFII-I primes numerous genomic loci during early embryogenesis.
- TFII-I binding frequently overlaps with H3K4me3/K27me3 bivalent chromatin at the promoters of developmental genes.
Conclusions:
- TFII-I plays a significant role in epigenetic mechanisms governing stem cell differentiation.
- Understanding TFII-I's function provides insights into developmental gene regulation.
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