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Population-based pediatric reference intervals for general clinical chemistry analytes on the Abbott Architect ci8200
Peter Ridefelt1, Mattias Aldrimer, Per-Olof Rödöö
1Department of Medical Sciences, Clinical Chemistry, Uppsala University, Uppsala, Sweden. peter.ridefelt@medsci.uu.se
Insights
This study establishes pediatric reference intervals for 12 common clinical chemistry analytes in children aged 6 months to 18 years. These age- and gender-specific values are essential for accurate diagnosis and treatment in pediatric healthcare.
Area of Science:
- Clinical Chemistry
- Pediatric Reference Intervals
- Diagnostic Benchmarks
Background:
- Reference intervals are critical for distinguishing healthy individuals from those with disease.
- Pediatric reference intervals are often unavailable or incomplete, hindering accurate clinical decisions.
- Establishing reliable reference intervals is vital for pediatric diagnostics.
Purpose of the Study:
- To define age- and gender-specific pediatric reference intervals for common clinical chemistry analytes.
- To provide essential data for improving diagnostic accuracy in children.
- To support clinical decision-making in pediatric healthcare settings.
Main Methods:
- Analyzed blood samples from 692 healthy children (6 months to 18 years).
- Measured 12 common general clinical chemistry analytes using the Abbott Architect ci8200 platform.
- Included sodium, potassium, chloride, calcium, albumin-adjusted calcium, phosphate, magnesium, creatinine (Jaffe and enzymatic), cystatin C, urea, and uric acid.
Main Results:
- Established age- and gender-specific pediatric reference intervals.
- Calculated reference intervals using the 2.5th and 97.5th percentiles.
- Provided a foundational dataset for pediatric clinical chemistry.
Conclusions:
- The generated reference intervals are specific to the Abbott Architect platform and a Caucasian population.
- Laboratories can utilize this data after local validation for their patient demographics.
- This study contributes crucial reference data for pediatric clinical chemistry.
Background:
Reference intervals are crucial decision-making tools aiding clinicians in differentiating between healthy and diseased populations. However, for children such values often are lacking or incomplete.
Methods:
Blood samples were obtained from 692 healthy children, aged 6 months to 18 years, recruited in daycare centers and schools. Twelve common general clinical chemistry analytes were measured on the Abbott Architect ci8200 platform; sodium, potassium, chloride, calcium, albumin-adjusted calcium, phosphate, magnesium, creatinine (Jaffe and enzymatic), cystatin C, urea and uric acid.
Results:
Age- and gender specific pediatric reference intervals were defined by calculating the 2.5th and 97.5th percentiles.
Conclusions:
The data generated is primarily applicable to a Caucasian population when using the Abbott Architect platform, but could be used by any laboratory if validated for the local patient population.
