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Updated: May 22, 2026
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A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
Published on: April 16, 2018
Cardiac-specific inducible and conditional gene targeting in mice
Thomas Doetschman1, Mohamad Azhar
1BIO5 Institute and Department of Cellular & Molecular Medicine, University of Arizona, Tucson, AZ, USA.
Circulation Research
|May 26, 2012
Summary
Mouse genetic engineering advances heart research by enabling conditional gene targeting and creating disease models. This review covers technologies and their limitations for cardiovascular research.
Area of Science:
- Cardiovascular Biology
- Genetics
- Developmental Biology
Background:
- Mouse genetic engineering is crucial for understanding heart development and disease.
- Conditional, tissue-specific, and temporal transgenic approaches are key technologies.
- Polymorphisms can be introduced into the mouse genome.
Purpose of the Study:
- To review genetic engineering technologies for cardiovascular research.
- To discuss the application of these technologies in studying heart homeostasis and pathophysiology.
- To highlight the development of clinically relevant cardiac disease models in mice.
Main Methods:
- Conditional tissue-specific and temporal transgenic approaches.
- Gene targeting strategies.
- Introduction of genetic polymorphisms.
Main Results:
- Genetic engineering has significantly advanced the understanding of cardiac molecular and genetic basis.
- These approaches are vital for elucidating genetic pathways in adult heart physiology and pathophysiology.
- Clinically relevant models of human cardiac diseases have been developed.
Conclusions:
- Genetic engineering technologies offer powerful tools for cardiovascular research.
- Understanding the limitations of these technologies is essential for effective application.
- Continued development and application will further unravel heart development and disease mechanisms.
