Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Key Techniques in Microbiology01:19

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Th17 plasticity and transition toward a pathogenic cytokine signature are regulated by cyclosporine after allogeneic SCT.

Blood advances·2018
Same author

Alcohol Consumption in Diabetic Patients with Nonalcoholic Fatty Liver Disease.

Canadian journal of gastroenterology & hepatology·2017
Same author

Attacking the mosquito on multiple fronts: Insights from the Vector Control Optimization Model (VCOM) for malaria elimination.

PloS one·2017
Same author

Concurrent validity of the Partners in Health scale against general self-rated health in chronic conditions: A short report.

Chronic illness·2017
Same author

Ion chemistry at elevated ion-molecule interaction energies in a selected ion flow-drift tube: reactions of H<sub>3</sub>O<sup>+</sup>, NO<sup>+</sup> and O<sub>2</sub><sup>+</sup> with saturated aliphatic ketones.

Physical chemistry chemical physics : PCCP·2017
Same author

Co-occurring medical conditions in adults with Down syndrome: A systematic review toward the development of health care guidelines.

American journal of medical genetics. Part A·2017

Related Experiment Video

Updated: May 22, 2026

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
08:46

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Implementing best practices and validation of cryopreservation techniques for microorganisms.

David Smith1, Matthew Ryan

  • 1Bioservices Team, Egham, CABI, Surrey TW20 9TY, UK. d.smith@cabi.org

Thescientificworldjournal
|May 26, 2012
PubMed
Summary

Maintaining authentic living organisms is crucial for life sciences and biotechnology research. Cryopreservation ensures the long-term stability and quality of biological resources, following best practices for research and industry.

More Related Videos

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

Minimum Volume Vitrification of Immature Feline Oocytes
07:16

Minimum Volume Vitrification of Immature Feline Oocytes

Published on: June 24, 2020

Related Experiment Videos

Last Updated: May 22, 2026

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
08:46

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

Minimum Volume Vitrification of Immature Feline Oocytes
07:16

Minimum Volume Vitrification of Immature Feline Oocytes

Published on: June 24, 2020

Area of Science:

  • Life Sciences
  • Biotechnology
  • Microbiology

Background:

  • Authentic living organisms are fundamental for research, industrial processes, and education.
  • Biological resource collections (BRCs) are essential for validating research and enabling future studies.
  • Maintaining the integrity of biological materials is critical for scientific reproducibility.

Purpose of the Study:

  • To outline best practices for maintaining and providing biological materials.
  • To review cryopreservation as an optimal method for long-term storage of microorganisms.
  • To discuss quality management in BRCs, focusing on fungal cryopreservation.

Main Methods:

  • Review of OECD best practice guidelines for BRCs.
  • Analysis of quality management options for biological resource maintenance.
  • Examination of cryopreservation techniques for fungi.

Main Results:

  • Cryopreservation is identified as a superior method for stable, long-term storage of microorganisms.
  • Best practice guidelines consolidate efforts from international organizations like OECD, CABRI, and WFCC.
  • The paper details methods to ensure reproducibility and quality in fungal cryopreservation.

Conclusions:

  • Adherence to established standards and best practices is vital for biological resource collections.
  • Effective cryopreservation techniques are key to preserving the full potential of fungal resources.
  • Standardized maintenance and provision of biological materials support robust scientific research and innovation.