Investigation of biological property of nanohydroxyapatite materials

The biological effects of nanohydroxyapatites and its cell toxicity have been studied using the MTT and ALP method, infrared spectrum of absorption and electrophoresis method, respectively. The nanohydroxyapatites are prepared and made by using sol-gel method, in which the parameters of process and reaction are controlled as: pH > 9, Ca/P = 1.67, sintering temperature of 1100 degrees C and sintering time 2 hours. Studied results show that nanohydroxyapatites can interact with human serum albumin and change its second structure and weight of molecules. We find that the nanohydroxyapatites and complex of nanoHAP + nanoCrO2 can all restrain the proliferation of MG63 cells, but their toxicities are first degree or minor, the toxicity of the complex is smaller than that of pure nanohydroxyapatites.