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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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Successful nanolitre real-time PCR detection of respiratory pathogens in clinical specimens

R Slinger1, I Moldovan, N Barrowman

  • 1Children's Hospital of Eastern Ontario, Ottawa, ON, Canada. slinger@cheo.on.ca

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|May 29, 2012
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...