[Fluorescent chromatogram profiles of uremic hemodialyzates and cirrhosis urines]

The unidentified fluorescence develop in body fluids during uremia and cirrhosis. We have used "hydrophobic" HPLC to profile these fluorescence in both 10 uremic hemodialyzates and 5 cirrhosis urines, and studied further to characterize the fluorescence and to gain more information of uremic fluorescent middle molecules. Chromatograms indicate evidently that the fluorescent substances are present in the middle molecule fraction of both hemodialyzates and urines. With reference to the chromatogram scale, the relative levels of fluorescent middle molecule in hemodialyzates were seemed 30% lower than the levels in urines, whereas the mean number of chromatogram peaks in hemodialyzates were 2-fold more than the number in urines. These unknown fluorescence have an excitation of 366 nm before and behind, and may produce more stable fluorescence of middle molecules by acid hydrolysis. These results suggest that there are possibly various modification on the uremic fluorescent substances.