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Related Experiment Video

Updated: May 22, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

Radiographic lymphangiography in the dog using iodized oil.

M N Mayer1, T I Silver, C K Lowe

  • 1Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada. monique.mayer@usask.ca

Veterinary and Comparative Oncology
|May 29, 2012
PubMed
Summary
This summary is machine-generated.

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This study compared direct and indirect lymphangiography in dogs for visualizing lymph nodes. Direct lymphangiography offered better contrast for certain nodes, while indirect methods were better for smaller vessels, with fewer side effects overall.

Area of Science:

  • Veterinary Radiology
  • Diagnostic Imaging
  • Lymphatic System Anatomy

Background:

  • Accurate visualization of lymph nodes is crucial for diagnosing various diseases in veterinary medicine.
  • Existing radiographic techniques for lymph node imaging have limitations in sensitivity and specificity.
  • The medial retropharyngeal, superficial cervical, axillary, superficial inguinal, and medial iliac lymph nodes are important targets for diagnostic evaluation.

Purpose of the Study:

  • To develop and evaluate clinically applicable radiographic techniques for visualizing specific canine lymph nodes.
  • To compare the efficacy and safety of direct versus indirect lymphangiography using iodized oil.
  • To determine the optimal lymphangiographic method for different lymph node groups.

Main Methods:

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Last Updated: May 22, 2026

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Published on: March 8, 2013

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  • 16 healthy research dogs were used to test direct and indirect lymphangiographic techniques.
  • Iodized oil was administered via both methods at multiple sites to enhance target lymph nodes.
  • Radiographs were taken to assess contrast uptake, lymphatic vasculature enhancement, and visualization quality.

    • Main Results:

    • Direct lymphangiography provided uniform contrast uptake and enhanced lymphatic vasculature for medial iliac and superficial cervical lymph nodes.
    • Indirect lymphangiography was more effective for visualizing smaller vessels in medial retropharyngeal, axillary, and superficial inguinal lymph nodes.
    • Indirect lymphangiography had a higher incidence of side effects (10/20 sites) compared to direct lymphangiography (3/16 sites).

    Conclusions:

    • Both direct and indirect lymphangiography are viable techniques for canine lymph node visualization, each with specific advantages.
    • Direct lymphangiography is recommended for larger nodes like medial iliac and superficial cervical.
    • Indirect lymphangiography is preferred for smaller, more distal lymph nodes, despite a higher rate of side effects.