Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
Pharmaceutical Poisoning: Treatment Strategies01:26

Pharmaceutical Poisoning: Treatment Strategies

Treatment strategies for poisoning are a critical aspect of emergency medicine, focusing on preventing the absorption of toxins and enhancing their elimination. When a poisoning incident occurs, the first response is to halt exposure and decontaminate the patient, particularly through gastrointestinal (GI) methods if the poison was ingested.Gastrointestinal Decontamination Techniques:Activated charcoal is the cornerstone of GI decontamination. It works through adsorption, binding the toxin to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glioma-intrinsic MAPK/ERK signaling promotes immunotherapy efficacy through T cell infiltration and interferon responses.

Nature communications·2026
Same author

Distinct transcription factors control tissue adaptation and effector function in infant and adult memory T cells.

Nature immunology·2026
Same author

Modular Design of Vacuum Systems for Lyophilization.

Industrial & engineering chemistry research·2026
Same author

Dissociable perfusion chip (DPC): perfusable microfluidic chip for single-cell screening of anti-cancer drugs in live glioblastoma explants.

Lab on a chip·2026
Same author

IDH-mutant inhibitors enhance the sensitivity of IDH1-mutant gliomas to cysteine-methionine deprivation and ferroptosis.

bioRxiv : the preprint server for biology·2026
Same author

Visualization and quantification of rDNA instabilities in mammalian cells and mouse models.

Nucleic acids research·2026

Related Experiment Video

Updated: May 22, 2026

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
07:13

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy

Published on: February 25, 2021

Rapid and robust detection methods for poison and microbial contamination.

Melanie M Hoehl1, Peter J Lu, Peter A Sims

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, USA. hoehl@mit.edu

Journal of Agricultural and Food Chemistry
|May 29, 2012
PubMed
Summary

Rapid, real-time monitoring for chemical and microbial contaminants in food, water, and medicine is now possible. A new instrument and methods detect contaminants like ethylene glycol and pathogens quickly and accurately on-site.

More Related Videos

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification
06:11

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Related Experiment Videos

Last Updated: May 22, 2026

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
07:13

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy

Published on: February 25, 2021

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification
06:11

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Environmental Science

Background:

  • High demand exists for real-time, on-site analyte monitoring in various products.
  • Current testing methods are often inadequate for widespread, rapid screening.

Purpose of the Study:

  • To introduce novel chemical methods for rapid quantification of chemical and microbial contaminants.
  • To develop a simple instrument for on-site, real-time analysis.

Main Methods:

  • Utilized a multichannel, multisample UV-vis spectrophotometer/fluorometer with simultaneous dual-frequency light interrogation.
  • Developed enzyme- and dye-based detection methods for specific analytes and pathogens.
  • Employed DNA intercalating dyes for pathogen detection in diverse matrices.

Main Results:

  • Successfully detected (di)ethylene glycol above 0.1 wt % and alcohols above 1 ppb without interference.
  • Achieved pathogen detection (E. coli, Salmonella, V. Cholera, Malaria model) above 10^4 CFU/mL in water, food, and blood.
  • Demonstrated universal scaling independent of pathogen size using multi-wavelength measurements.
  • Contaminants detected directly without prior separation, purification, concentration, or incubation.
  • Developed chemistry stable for over 3 weeks without refrigeration, with measurements under 5 minutes.

Conclusions:

  • The developed methods and instrument enable rapid, on-site detection of a wide range of chemical and microbial contaminants.
  • This technology offers a significant advancement for ensuring safety in food, water, medicines, and household products.
  • The system provides a versatile and stable solution for real-time monitoring needs.