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Array-based functional screening of heparin glycans.

Tania M Puvirajesinghe¹, Yassir A Ahmed, Andrew K Powell

¹Centre for Glycobiology, Department of Biochemistry and Cell Biology, Institute of Integrative Biology, The University of Liverpool, Liverpool L69 7ZB, UK.

Chemistry & Biology

|May 29, 2012

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This study introduces a novel glycobioarray for studying cell responses to heparin saccharides. The platform measures fibroblast growth factor (FGF) signaling activation, enabling functional glycomics screening.

Area of Science:

Glycoscience
Cellular signaling
Biotechnology

Background:

Array methodologies are vital for glycan-protein interaction studies.
Existing methods lack the ability to generate crucial cell response data.
This gap limits comprehensive functional glycomics analysis.

Purpose of the Study:

To develop a novel slide-based array platform for measuring cell responses to immobilized glycans.
To demonstrate the platform's utility by assessing fibroblast growth factor (FGF) signaling activation.
To establish a tool for functional glycomics screening.

Main Methods:

Development of a slide-based array with immobilized heparin saccharides.
Overlaying heparan sulfate-deficient Swiss 3T3 cells onto the array.

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Stimulation with FGF2 and assessment of downstream ERK1/2 phosphorylation via immunofluorescence.

Main Results:

Cellular activation of FGF signaling was observed.
Activation correlated with specific heparin saccharides capable of activating FGF signaling.
Differential activation by various heparin saccharide sizes was quantitatively measured.

Conclusions:

The developed glycobioarray platform enables the measurement of cell signaling responses to glycans.
This method provides a powerful tool for functional glycomics.
The platform has significant potential for broad applications in glycan-protein interaction research.