Related Experiment Video
Updated: May 22, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Polymerase incorporation of pyrene-nucleoside triphosphates
Marcel Hollenstein1, Filip Wojciechowski, Christian J Leumann
1Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland. hollenstein@dcb.unibe.ch
Abstract:
Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.
More Related Videos
Related Concept Videos
Biosynthesis of Nucleic Acids
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Proofreading
PCR
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...

