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Updated: May 22, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Preparation of DNA-polyintercalator conjugate and its functional property
Masanori Yamada1, Ayaka Okumoto, Tetsuya Yamada
1Department of Chemistry, Faculty of Science, Okayama University of Science, Ridaicho, Kita-Ku, Okayama 700-0005, Japan. myamada@chem.ous.ac.jp
Abstract:
Psoralen immobilized polyvinyl alcohol (PVA-P) was synthesized from chloromethylmethoxypsoralen and polyvinyl alcohol. The psoralen part of PVA-P intercalated into the double-stranded DNA and formed covalent bonding between the psoralen and nucleic acid base after 365 nm UV irradiation. As a result, DNA and PVA-P produced a water-insoluble conjugate. This DNA-PVA-P conjugate maintained the double-stranded structure of DNA and possessed the DNA's property, such as intercalation. Therefore, the DNA-PVA-P conjugate selectively accumulated the planar-structure containing chemical compounds, such as biphenyl and dibenzofuran, from an aqueous multi-component solution. These DNA-PVA-P conjugates may have the potential to be utilized as a separation material for the selective removal of harmful compounds.
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