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Reference intervals for reproductive hormones in prepubertal children on the automated Roche cobas e 411 analyzer
Ivana Zec1, Ivana Kučak, Ilijana Begčević
1Laboratory of Endocrinology, Clinics of Oncology and Nuclear Medicine, University Hospital Center Sestre milosrdnice, Vinogradska 29, 10000 Zagreb, Croatia.
Objectives:
To establish reference intervals for luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2), progesterone (P), total and free testosterone (T) and sex-hormone binding globulin (SHBG) in prepubertal children and to assess age- and gender-related differences.
Design And Methods:
A total of 948 subjects, 480 girls and 468 boys, between 1 and 11 years of age, were included in this study. All assays were performed on a Roche cobas e 411 immunoassay analyzer. Reference intervals have been evaluated according to the most recent CLSI guidelines.
Results:
Median values of LH, FSH and T were significantly higher in subgroups ranging from ≥ 8 to < 11 years, for both genders. In girls of that age, reference values of E2 were significantly higher than in younger ones, and in boys of the corresponding age.
Conclusion:
Established reference intervals are applicable to other laboratories that use the same instrumentation.