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The salt-responsive transcriptome of Populus simonii × Populus nigra via DGE
Su Chen1, Jing Jiang, Huiyu Li
1State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University), 26 Hexing Road, Harbin, China
Abstract:
In this study, the dynamic transcriptome of poplar (Populus simonii × Populus nigra) was investigated under salt stress using Solexa/illumine digital gene expression (DGE) technique. A total of 5453, 2372, and 1770 genes were shown to be differentially expressed after exposure to NaCl for 3 days, 6 days and 9 days, respectively. Differential expression patterns throughout salt stress were identified for 572 genes. Gene ontology classification analysis of these differentially expressed genes revealed that numerous genes mapped to "transporter activity" and "response to stress". The dynamic transcriptome expression profiles of poplar under salt stress obtained in this study may provide useful insights for further analysis of the mechanism of high salinity tolerance in plants. Furthermore, these differentially expressed genes under salt stress may allow identification of potential genes as suitable targets for biotechnological manipulation with the aim of improving poplar salt tolerance.
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02:02Responses to Salt Stress
01:10Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...