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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

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Published on: September 9, 2012

Antibodies to prothrombin.

M L Bertolaccini¹

¹Lupus Research Unit, The Rayne Institute, King's College London, St Thomas' Hospital, London, UK. maria.bertolaccini@kcl.ac.uk

|May 29, 2012

Summary

Research shows antiprothrombin (aPT) and aPS/PT antibodies are linked to antiphospholipid syndrome (APS) clinical features. Though distinct, these autoantibodies can coexist and are being investigated for their role in lupus anticoagulant.

Area of Science:

Immunology
Hematology
Rheumatology

Background:

Antiphospholipid antibodies (aPL) research has expanded since the 1980s.
Antiprothrombin (aPT) and aPS/PT antibodies are key autoantibodies in antiphospholipid syndrome (APS).

Purpose of the Study:

To investigate the association and correlation between aPT and aPS/PT antibodies.
To explore the role of these antibodies in antiphospholipid syndrome (APS) and lupus anticoagulant (LA).

Main Methods:

Enzyme-linked immunosorbent assay (ELISA) was used for detecting aPT antibodies.
Irradiated plates and prothrombin in complex with phosphatidylserine (aPS/PT) were utilized for antibody detection.

Main Results:

aPT and aPS/PT antibodies are associated with APS-related clinical features.

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These distinct autoantibody populations can be present concurrently in patients.

Conclusions:

aPT and aPS/PT antibodies, while distinct, correlate with each other and APS.
Further investigation is needed to understand their role as potential antigenic targets for lupus anticoagulant (LA).