Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Data Validation

Data Validation

Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:

Bioequivalence Data: Statistical Interpretation

Bioequivalence Data: Statistical Interpretation

The statistical interpretation of bioequivalence data is a significant aspect of pharmaceutical research. Bioequivalence refers to the absence of any significant difference in the rate and extent to which the active ingredient in pharmaceutical products becomes available at the site of drug action when administered at the same molar dose under similar conditions. This helps determine if different drug products have similar absorption rates, ensuring their interchangeability.Statistical...

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess the...

Sensitivity, Specificity, and Predicted Value

Sensitivity, Specificity, and Predicted Value

In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...

Clinically Relevant Drug Product Specifications: Methods of Establishment

Clinically Relevant Drug Product Specifications: Methods of Establishment

Product specifications define the acceptable quality of a pharmaceutical product by ensuring identity, purity, potency, and strength. These specifications serve as benchmarks during development, manufacturing, and post-approval quality control. Clinically relevant specifications are particularly important because they directly relate to a drug's safety and efficacy in clinical use.Dissolution studies are critical biopharmaceutic tools that link in vitro behavior to in vivo performance. They...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cardiac troponin interferents in diagnosis and clinical management of myocarditis: a single center experience.

International journal of cardiology·2025

Same author

Probing Exotic Cross-Shell Interactions at N=28 with Single-Neutron Transfer on ^{47}K.

Physical review letters·2025

Same author

The safety of available pharmacotherapy for stroke prevention in atrial fibrillation.

Expert opinion on drug safety·2024

Same author

Measurement of the Gamma-Ray-to-Neutron Branching Ratio for the Deuterium-Tritium Reaction in Magnetic Confinement Fusion Plasmas.

Physical review letters·2024

Same author

Development of a measuring technique based on JET second D-T campaign (DTE2) experience for assessing fusion power at ITER during D-T operation using the radial gamma-ray spectrometer.

The Review of scientific instruments·2024

Same author

High-Precision Spectroscopy of ^{20}O Benchmarking Ab Initio Calculations in Light Nuclei.

Physical review letters·2024

Search research articles

Related Experiment Video

Updated: May 22, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Interpretation of laboratory data and need for reference laboratories.

V Pengo¹, G Denas, A Banzato

¹Clinical Cardiology, Thrombosis Centre, Department of Cardiac Thoracic and Vascular Sciences, University of Padua, Italy. vittorio.pengo@unipd.it

|May 29, 2012

Summary

A single positive antiphospholipid antibody test does not predict clotting events or antiphospholipid syndrome. However, multiple positive tests, especially triple positivity, strongly indicate high risk for thromboembolic events.

More Related Videos

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Related Experiment Videos

Last Updated: May 22, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Area of Science:

Immunology
Hematology
Clinical Pathology

Background:

Antiphospholipid antibodies (aPL) are associated with an increased risk of thrombosis.
Antiphospholipid Syndrome (APS) diagnosis requires specific clinical and laboratory criteria.
The diagnostic and prognostic significance of a single positive aPL test versus multiple positive tests is not fully elucidated.

Purpose of the Study:

To evaluate the association between the number of positive antiphospholipid antibody tests and thromboembolic events.
To determine if antiphospholipid antibody positivity identifies patients at high risk for antiphospholipid syndrome.
To investigate the role of specific antibodies, such as anti-Domain I, in predicting adverse outcomes.

Main Methods:

Retrospective analysis of laboratory test results for antiphospholipid antibodies.
Correlation of laboratory findings with the occurrence of thromboembolic events.
Identification of specific antiphospholipid antibodies in patients with positive test results.

Main Results:

A single positive antiphospholipid antibody test was not associated with thromboembolic events.
Multiple positive antiphospholipid antibody tests, particularly triple positivity, showed a strong association with thromboembolic events.
Triple positivity was linked to the presence of anti-Domain I antibodies, which can prolong coagulation tests.

Conclusions:

The number of positive antiphospholipid antibody tests is crucial for risk stratification.
Triple positivity for antiphospholipid antibodies identifies patients at high risk for thromboembolic events.
Anti-Domain I antibodies may play a key role in the pathogenesis of antiphospholipid syndrome and could be targets for assay standardization.