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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: May 22, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Linear motifs confer functional diversity onto splice variants.

Robert J Weatheritt1, Norman E Davey, Toby J Gibson

  • 1Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg 69117, Germany.

Nucleic Acids Research
|May 29, 2012
PubMed
Summary

Alternative splicing and promoter usage create protein diversity through messenger RNA (mRNA) modifications. This study reveals that alternative exons are rich in linear motifs, crucial for regulating protein interactions and generating functional diversity.

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Related Experiment Videos

Last Updated: May 22, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Messenger ribonucleic acid (mRNA) modifications like alternative promoter usage and alternative splicing generate significant protein diversity, a phenomenon known as pleiotropy.
  • The functional consequences of this dynamically generated diversity remain incompletely understood despite extensive research.

Purpose of the Study:

  • To investigate the role of protein interaction modules within alternative exons and regions affected by alternative promoter usage.
  • To determine how these variations contribute to protein isoform diversity and regulatory mechanisms.

Main Methods:

  • Analysis of protein modules, focusing on intrinsically disordered regions (IDRs), within alternative exons.
  • Examination of the enrichment of specific linear motifs (e.g., PDZ-, PTB-, SH2-, WW-domain binding motifs) in alternatively spliced regions.
  • Assessment of intrinsically disordered regions (IDRs) in segments affected by alternative promoter usage.

Main Results:

  • Alternative exons are significantly enriched in linear motifs, indicating their importance in regulating protein interactions.
  • Specific motifs, including those binding to PDZ, PTB, SH2, and WW domains, are more prevalent in alternative exons.
  • Regions modified by alternative promoter usage show enrichment in intrinsically disordered regions (IDRs), linking protein isoform diversity to IDR modulation.

Conclusions:

  • Short linear motifs are critical components in generating protein diversity among splice variants.
  • The inclusion or removal of exons containing specific interaction modules is a key regulatory mechanism.
  • Protein isoform diversity is closely associated with the modulation of intrinsically disordered regions (IDRs).