Related Experiment Video
Updated: May 22, 2026
07:12
Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
Published on: August 23, 2011
Pressure cycling technology in systems biology
Bradford S Powell1, Alexander V Lazarev, Greta Carlson
1Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. bpowell@mris.com
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2012
Summary
Pressure cycling technology (PCT) offers a novel solution for systems biology sample preparation. This method enables comprehensive analyte extraction and safe sterilization, overcoming limitations of traditional techniques for complex or pathogenic samples.
Area of Science:
- Systems Biology
- Biotechnology
- Analytical Chemistry
Background:
- Traditional sample preparation methods limit analyte extraction from single samples.
- Technical constraints and sample volume often necessitate using multiple replicate experiments.
- Integrating data from disparate sample preparation streams increases uncertainty and confounds biological system understanding.
Purpose of the Study:
- To introduce ultra-high pressure cycling technology (PCT) as an enabling sample preparation strategy for systems biology.
- To highlight PCT's advantages in overcoming limitations of traditional methods for complex and pathogenic samples.
- To provide procedures and findings for incorporating PCT into preanalytical sample treatment.
Main Methods:
- Utilizing ultra-high pressure cycling technology (PCT), also known as barocycling.
- Applying PCT for uniform disruption of sample matrices.
- Employing a high-pressure solvent environment during sample preparation.
Main Results:
- PCT allows for the extraction of a more complete set of analytes from a single sample.
- Samples prepared with PCT show no degradation of target biomolecules.
- PCT safely sterilizes complex or pathogenic viral, bacterial, and spore samples.
Conclusions:
- PCT is a versatile preanalytical sample treatment method for systems biology.
- This technology overcomes analyte-specific commitments and material degradation issues.
- PCT facilitates comprehensive and reliable data integration for a better understanding of biological systems.
Related Concept Videos
01:27ATP Driven Pumps I: An Overview
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
01:34ATP Driven Pumps II: P-type Pumps
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
01:27Fast Reactions
Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...
01:30ATP Driven Pumps III: V-type Pumps
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
01:58Mechanical Protein Functions
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.