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Related Experiment Video

Updated: May 22, 2026

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

Microbial proteomics using mass spectrometry.

Harry B Hines¹

¹Integrated Toxicology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA. harry.hines@amedd.army.mil

Methods in Molecular Biology (Clifton, N.J.)

|May 29, 2012

Summary

This summary is machine-generated.

This study outlines core proteomic techniques applicable across various subjects, focusing on bacterial proteomic analysis. It presents a quantitative method, discussing its potential, challenges, and emerging technologies for bacterial investigations.

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Area of Science:

Proteomics
Microbiology
Mass Spectrometry

Background:

Proteomic analyses comprise complex, interconnected steps requiring careful coordination for optimal data acquisition.
Many proteomic steps are universal, adaptable to different study objectives with minor modifications.
This universality forms a foundational set of proteomic approaches applicable to diverse research subjects.

Purpose of the Study:

To provide an overview of core proteomic approaches, techniques, and instrumentation.
To illustrate the application of these proteomic concepts and techniques specifically to bacterial investigations.
To present a practical, quantitative method for bacterial proteomic analysis, including potential issues and advancements.

Main Methods:

Overview of fundamental proteomic strategies and mass spectrometry instrumentation.
Adaptation and application of general proteomic techniques for bacterial studies.
Development and presentation of a quantitative method for bacterial proteomic analysis.

Main Results:

Identification of common proteomic steps applicable to most research subjects.
Demonstration of how core proteomic techniques can be tailored for bacterial investigations.
Presentation of a quantitative method with an analysis of its feasibility, limitations, and future technological prospects.

Conclusions:

Core proteomic methodologies provide a versatile framework for diverse biological studies.
Specific adaptations enable the effective application of proteomic analysis to bacterial systems.
The presented quantitative method offers a valuable tool for bacterial proteomic research, highlighting areas for improvement and innovation.