Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 22, 2026

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

Analysis of 3D branching pattern: hematoxylin and eosin method.

Sunder Sims-Lucas¹

¹Children's Hospital of Pittsburgh, Rangos Research Institute, Pittsburgh, PA, USA. Sunder.Sims-Lucas@chp.edu

Methods in Molecular Biology (Clifton, N.J.)

|May 29, 2012

Summary

This study introduces a novel 3D reconstruction method for developing kidneys, enabling detailed analysis of organ development and structural abnormalities. This technique aids in understanding kidney developmental defects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mouse Model of Acute to Chronic Kidney Disease Transition Induced by Renal Ischemia/Reperfusion Injury.

Journal of visualized experiments : JoVE·2026

Same author

Novel high-resolution ion mobility mass spectrometry for site-specific quantification of the sirtuin-5 regulated kidney succinylome.

Journal of proteomics·2026

Same author

Novel high-resolution ion mobility mass spectrometry for site-specific quantification of the sirtuin-5 regulated kidney succinylome.

bioRxiv : the preprint server for biology·2025

Same author

Mitochondrial organization in the developing proximal tubule is controlled by LRRK2.

Nature communications·2025

Same author

Mitochondrial organization in the developing proximal tubule is controlled by LRRK2.

bioRxiv : the preprint server for biology·2025

Same author

Loss of long-chain acyl-CoA dehydrogenase protects against acute kidney injury.

JCI insight·2025

Area of Science:

Developmental Biology
Organogenesis
Biomedical Imaging

Background:

Understanding developmental defects requires accurate analysis of organ architecture.
Three-dimensional (3D) analysis is crucial for linking structural abnormalities to developmental issues.

Purpose of the Study:

To describe a novel 3D reconstruction technique for the developing kidney.
To enable quantitative analysis of kidney structures and their architecture.

Main Methods:

Serial sectioning of developing kidneys.
Histological staining and digital projection of serial images.
Tracing of tissue lineages and structures for 3D rendering and quantitative analysis.

Main Results:

More Related Videos

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

Published on: August 2, 2013

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Related Experiment Videos

Last Updated: May 22, 2026

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

Published on: August 2, 2013

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Successful 3D reconstruction of the developing kidney, including capsule, ureteric epithelium, and nephrons.
Demonstrated quantitative analysis of reconstructed tissues (surface area, volume).
Skeletonization of ureteric epithelium to analyze branching architecture.

Conclusions:

The developed 3D reconstruction technique provides a powerful tool for studying kidney development.
This method facilitates the quantitative assessment of structural changes and developmental defects.
Enables detailed analysis of ureteric tree branching patterns in the developing kidney.