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Related Experiment Video

Updated: May 22, 2026

Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

Published on: May 22, 2019

Estimating nephron number in the developing kidney using the physical disector/fractionator combination.

Luise A Cullen-McEwen¹, James A Armitage, Jens R Nyengaard

¹Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|May 29, 2012

Summary

This summary is machine-generated.

A new histochemical/stereological technique enables accurate estimation of nephron number in developing kidneys. This method is crucial for understanding kidney development and disease in young mammals.

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Area of Science:

Nephrology
Developmental Biology
Stereology

Background:

Design-based stereology is the gold standard for adult kidney nephron counting.
A design-based stereology method for developing kidneys was previously unavailable.
Accurate estimation requires unambiguous identification of developing nephrons.

Purpose of the Study:

To present a novel histochemical/stereological technique for estimating total nephron number in the developing kidney.
To enable precise quantification of nephrogenesis in young mammals.

Main Methods:

Combined histochemical staining and stereological principles.
Application to developing mouse and rat kidneys.
Adaptable methodology for other species.

Main Results:

Successful implementation of a new method for estimating nephron number in developing kidneys.
Provides accurate and precise estimates essential for developmental studies.

Conclusions:

The described technique provides a robust method for quantifying nephron number during kidney development.
This advancement is vital for research into kidney development and related pathologies.