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Updated: May 22, 2026

08:07
Simple Detection of Primary Cilia by Immunofluorescence
Published on: May 15, 2020
Investigating primary cilia in cultured metanephric mesenchymal cells
1Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2012
Abstract:
Primary cilia are present in most vertebrate cells. They have complex structures that are required for signal transduction in developing tissues. The embryonic kidney consists of two major cell lineages, ureteric and metanephric mesenchyme. Here, we describe a method to isolate metanephric mesenchyme from ureteric bud, culture metanephric mesenchyme cells, and study primary cilia in cell culture.

