Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coagulation impact on chemotactic activity generation for polymorphonuclear leukocytes.

A Morin1, M M Arvier, F Doutremepuich

  • 1Centre de Transfusion Sanguine, Vandoeuvre, France.

Thrombosis Research
|September 15, 1990
PubMed
Summary

Human alpha thrombin attracts polymorphonuclear leukocytes, a key immune cell, to sites of coagulation. This chemotactic effect is concentration-dependent, highlighting thrombin's role in immune cell recruitment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neural correlates of foreign accent syndrome: Is white matter the key to the mystery?

Revue neurologique·2025
Same author

Causes, management, and prognosis of severe gastrointestinal bleedings in critically ill patients with pancreatic cancer: A retrospective multicenter study.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2024
Same author

Neurological outcome of cardiac arrest patients in mountain areas: An analysis of the Northern French Alps Emergency Network.

The American journal of emergency medicine·2024
Same author

Ptosis secondary to orbital metastasis undetected by magnetic resonance imaging: A case report.

Journal francais d'ophtalmologie·2024
Same author

The impact of patients as trainers on registered nurses' patient engagement in primary care clinics: a qualitative study.

BMC primary care·2023
Same author

Psychiatric burden in a cohort of adults with Niemann Pick type C disease: from psychotic symptoms to frontal lobe behavioral disorders.

Orphanet journal of rare diseases·2023

Area of Science:

  • Biochemistry
  • Immunology
  • Hematology

Background:

  • Polymorphonuclear leukocytes (PMNs) are crucial for innate immunity and inflammation.
  • Thrombin, a key enzyme in the coagulation cascade, plays roles beyond hemostasis.
  • The specific chemoattractant properties of alpha thrombin for PMNs require elucidation.

Purpose of the Study:

  • To investigate the chemotactic activity of human alpha thrombin on human polymorphonuclear leukocytes.
  • To determine the concentration range for optimal alpha thrombin-induced PMN chemotaxis.
  • To compare the chemotactic potential of alpha thrombin versus its precursor, human prothrombin.

Main Methods:

  • Chemotaxis assay using agarose gel.
  • Testing of varying concentrations of purified human alpha thrombin.

Related Experiment Videos

  • Testing of human prothrombin at similar concentrations.

    • Main Results:

    • Human alpha thrombin exhibits significant chemoattractant properties for human polymorphonuclear leukocytes.
    • Optimal chemotaxis occurs between 1.4 x 10^-5 M and 1.6 x 10^-5 M, with activity observed from 2.8 x 10^-6 M to 2.2 x 10^-5 M.
    • Human prothrombin demonstrated no chemotactic activity on polymorphonuclear leukocytes within the tested concentration range.

    Conclusions:

    • Alpha thrombin acts as a direct chemoattractant for polymorphonuclear leukocytes.
    • This finding suggests a role for alpha thrombin in recruiting immune cells to sites of vascular injury and coagulation.
    • The specific activation of PMNs by alpha thrombin during coagulation warrants further investigation.