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Published on: July 1, 2018
[The diagnosis of Eperythrozoon suis infection]
1II. Medizinischen Tierklinik, Ludwig-Maximilians-Universität München.
Abstract:
Eperythrozoon infection can be diagnosed clinically and hematologically only during acute attacks. In the febrile phase it is possible to detect the eperythrozoon organisms in the blood smear. The best method for staining is that by acridine-orange and examination by fluorescence microscopy. Serological identification (IHA, KBR, ELISA) is suitable for herd diagnosis only, because of the undulating level of the titre. In the individual case only positive results are regarded as significant. The best method to diagnose a latent infection of eperythrozoon suis is to splenectomize a suspected pig. The infection with eperythrozoon suis is a herd problem, therefore an examination should be made whenever there are pale pigs with fever, or a reduction of efficiency or an increased susceptibility to other infections.
Insights
Diagnosing Eperythrozoon infection in pigs requires specific methods. Acridine-orange staining and fluorescence microscopy are best for acute cases, while serological tests aid herd diagnosis.
Area of Science:
- Veterinary Hematology
- Microbiology
- Animal Pathology
Context:
- Eperythrozoon suis infections present diagnostic challenges, particularly in distinguishing acute from latent stages.
- Clinical and hematological signs are often only apparent during acute febrile episodes.
Purpose:
- To outline effective diagnostic strategies for Eperythrozoon suis infections in swine.
- To differentiate between methods suitable for individual diagnosis versus herd-level screening.
Summary:
- Acute Eperythrozoon infections are detectable in blood smears during fever, with acridine-orange staining and fluorescence microscopy being optimal detection methods.
- Serological assays like indirect hemagglutination assay (IHA), complement fixation test (KBR), and enzyme-linked immunosorbent assay (ELISA) are valuable for herd diagnosis due to fluctuating antibody titers.
- Splenectomy is identified as the most effective method for diagnosing latent Eperythrozoon suis infections in individual pigs.
Impact:
- Improved diagnostic accuracy for Eperythrozoon suis, leading to better herd management strategies.
- Facilitates early detection and intervention, potentially reducing economic losses associated with reduced pig efficiency and increased susceptibility to secondary infections.
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