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Updated: May 21, 2026

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
12:38

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

Published on: February 15, 2018

[Analysis of titration curves of serum antibodies obtained by ELISA]

S A Bobrovnik

    Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
    |May 31, 2012
    PubMed

    Different forms of titration curves obtained by ELISA for serum antibodies, monoclonal antibodies and polyreactive immunoglobulins were considered. A new interpretation of dose-dependent titration curves was suggested. It was shown that our interpretation of dose-dependent titration curves for antibodies which are presented in some biological liquids allow obtaining additional information about properties of the samples. This information was not obtained earlier because of the wrong understanding of the considered problem.

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