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Related Experiment Video

Updated: May 21, 2026

Murine Superficial Lymph Node Surgery
04:36

Murine Superficial Lymph Node Surgery

Published on: May 21, 2012

Murine superficial lymph node surgery.

Mélissa Mathieu1, Nathalie Labrecque

  • 1Maisonneuve-Rosemont Hospital Research Center.

Journal of Visualized Experiments : Jove
|May 31, 2012
PubMed
Summary
This summary is machine-generated.

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Sequential lymph node removal in mice allows researchers to track immune responses over time within the same animal. This method provides ample cells for analysis, reducing variability and animal use in immunology studies.

Area of Science:

  • Immunology
  • Cellular immunology
  • Experimental immunology

Background:

  • Understanding immune responses over time is crucial for vaccine, infection, and tumor studies.
  • Lymphocyte homeostasis research also necessitates time-course experiments.
  • Studying circulating and lymph node-resident lymphocytes requires examining both compartments.

Purpose of the Study:

  • To describe a minimally invasive surgical technique for sequential lymph node removal in mice.
  • To enable longitudinal studies of immune cell populations within the same animal.
  • To provide a method for obtaining sufficient cells for detailed immunological analysis.

Main Methods:

  • A technique for sequential brachial and inguinal lymphadenectomy in anesthetized mice is detailed.

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Murine Bilateral Renal Lymphadenectomy
06:31

Murine Bilateral Renal Lymphadenectomy

Published on: December 30, 2025

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Last Updated: May 21, 2026

Murine Superficial Lymph Node Surgery
04:36

Murine Superficial Lymph Node Surgery

Published on: May 21, 2012

Murine Bilateral Renal Lymphadenectomy
06:31

Murine Bilateral Renal Lymphadenectomy

Published on: December 30, 2025

  • The superficial and rapid surgical procedure ensures quick mouse recovery and minimal pain.
  • Lymph nodes can be harvested every two days for time-course experiments.

    • Main Results:

    • The described lymphadenectomy technique yields 1-2x10^6 cells per lymph node.
    • This cell yield is sufficient for phenotypic characterization and functional assays.
    • The method allows for the study of lymph node-resident lymphocytes over time in vivo.

    Conclusions:

    • Sequential lymph node surgery is a valuable tool for time-course immunology research.
    • This technique reduces experimental variability and the number of animals required.
    • The approach is adaptable to various experimental designs in immunological studies.